| 利用tyrB与aspA基因在大肠杆菌中的偶联表达制备L-苯丙氨酸 |
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| 引用本文： | 李欣,范长胜,王海蛟,高山峨,梁国新,陶菊红.利用tyrB与aspA基因在大肠杆菌中的偶联表达制备L-苯丙氨酸[J].复旦学报(自然科学版),2005,44(6):1047-1050. |
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| 作者姓名： | 李欣 范长胜 王海蛟 高山峨 梁国新 陶菊红 |
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| 作者单位： | 复旦大学,生命科学学院,微生物学和微生物工程系,上海,200433;复旦大学,生命科学学院,微生物学和微生物工程系,上海,200433;复旦大学,生命科学学院,微生物学和微生物工程系,上海,200433;复旦大学,生命科学学院,微生物学和微生物工程系,上海,200433;复旦大学,生命科学学院,微生物学和微生物工程系,上海,200433;复旦大学,生命科学学院,微生物学和微生物工程系,上海,200433 |
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| 基金项目： | The project supported by the National Natural Science Foundation of China （No. 30470045） |
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| 摘 要： | 报道了利用tyrB与aspA串联表达来合成苯丙氨酸．首先将抄当与aspA串联在质粒pBV221上，构成串联表达质粒pBV-tyrB-aspA；然后将该重组质粒转化到E．coli K36菌株中表达．对基因编码的相关酶活性以及工程菌利用FA和PPA生成Phe进行研究，结果表明：利用AspA和TyrB的偶联反应可以有效的提高E．coli K36合成L-苯丙氨酸的能力．
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| 关 键 词： | 代谢工程 tyrB aspA 苯丙氨酸 大肠杆菌 |
| 文章编号： | 0427-7104（2005）06-1047-04 |
| 收稿时间： | 04 22 2005 12:00AM |
| 修稿时间： | 2005-04-22 |
Phenylalanine Synthesis by Coupling tyrB-aspA Expression in Escherichia coli |
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| LI Xin,FAN Chang-sheng,WANG Hai-jiao,GAO Shan-e,LIANG Guo-xin,TAO Ju-hong.Phenylalanine Synthesis by Coupling tyrB-aspA Expression in Escherichia coli[J].Journal of Fudan University(Natural Science),2005,44(6):1047-1050. |
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| Authors: | LI Xin FAN Chang-sheng WANG Hai-jiao GAO Shan-e LIANG Guo-xin TAO Ju-hong |
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| Institution: | Department of Microbiology and Microbial Biotechnology , School of Life Sciences, Fudan University, Shanghai 200433, China |
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| Abstract: | To improve productivity,a special biosynthetic route has been applied in the phenylalanine-producing strain Escherichia coli K36.By coupling tyrB and aspA,the biosynthesis of phenylalanine was completed in the reaction solution containing compounds PPA,FA and NH~+_4.Here,gene tyrB and aspA of E.coli K12 were inserted into plasmid pBV221 to construct a recombinant plasmid pBV-tyrB-aspA,and it was introduced into E.coli K36.The enzymatic activities of AspA and TyrB were measured and the phenylalanine productivities of the recombinant strains were analyzed.The results demonstrated that co-expression of tyrB and(aspA) was able to enhance phenylalanine biosynthesis. |
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| Keywords: | tyrB aspA metabolic engineering tyrB aspA phenylalanine Escherichia coli |
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