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| 黒腹果蝇黑条体突变型蛋白质组分析中二维凝胶电泳技术的改进 | |
| 引用本文： | 严镇钧,徐玲花,曾庆韬,金珊.黒腹果蝇黑条体突变型蛋白质组分析中二维凝胶电泳技术的改进[J].湖北师范学院学报(自然科学版),2008,28(4). |
| 作者姓名： | 严镇钧 徐玲花 曾庆韬 金珊 |
| 作者单位： | 1. 湖北大学,生命科学学院,湖北,武汉,430062;湖北师范学院,生命科学学院,湖北,黄石,435002 2. 黄石理工学院化学与材料工程学院,湖北,黄石,435000 3. 湖北大学,生命科学学院,湖北,武汉,430062 |
| 摘 要： | 用黒腹果蝇黑条体突变型为材料,介绍一种改进的蛋白质组分析中的二维凝胶电泳技术。该方法在电解质的配比以及银染过程等方面对传统的二维凝胶电泳技术加以了改进,不仅缩短了实验时间,减轻了实验工作量,而且得到了更好的实验效果;并且该方法对实验条件要求不高,在普通的实验室条件下即可被运用。 |
| 关 键 词： | 果蝇 黑条体 蛋白质组 二维凝胶电泳 |
Improvement in the two-dimensional gel electrophoresis of proteins from the mutant of black streak of D.melanogaster |
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| YAN Zhen-jun,XU Ling-hua,ZENG Qing-tao,JIN Shan.Improvement in the two-dimensional gel electrophoresis of proteins from the mutant of black streak of D.melanogaster[J].Journal of Hubei Normal University(Natural Science),2008,28(4). | |
| Authors: | YAN Zhen-jun XU Ling-hua ZENG Qing-tao JIN Shan |
| Institution: | 1(1.College of Life Science; Hubei University; Wuhan 430062; China; 2.College of Life Science; Hubei Normal University; Huangshi 435002; 3.School of Chemical and Material Engineering; Huangshi Institute of Technology; Huangshi 435000; China); |
| Abstract: | The mutant of black streak of D.melanogaster is used to introduce a new method of the Two-dimensional Gel Electrophoresis of Proteins.In this paper,the proportion of electrolytes and silver staining of conventional method is improved,the new method makes us spend less time and labor getting the better result,and has a low demand for manipulation,so it can be applied in the laboratory under simple conditions. |
| Keywords: | D melanogaster black streak proteins two-dimensional gel electrophoresis |
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