| 柚皮苷与人血清蛋白作用机理探索 |
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| 引用本文： | 王玲,赵俊宏,杨冉.柚皮苷与人血清蛋白作用机理探索[J].河南科学,2014(5):716-718. |
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| 作者姓名： | 王玲 赵俊宏 杨冉 |
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| 作者单位： | [1]郑州师范学院化学系,郑州450044 [2]河南省科学院化学研究所有限公司,郑州450002 [3]郑州大学化学与分子工程学院,郑州450052 |
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| 基金项目： | 河南省教育厅基金资助项目（128150031） |
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| 摘 要： | 利用荧光光谱及同步荧光光谱法研究了柚皮苷与人血清蛋白相互作用.结果表明,相互作用使得人血清蛋白荧光猝灭且引起蛋白构象改变;猝灭常数大小证明人血清蛋白荧光猝灭机制为静态猝灭;根据能量转移原理求得结合距离为3.7 nm,存在发生能量转移的可能性.
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| 关 键 词： | 人血清蛋白 柚皮苷 荧光光谱 作用机理 |
The Interaction Between Naringin and Human Serum Albumin |
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| Wang Ling,Zhao Junhong,Yang Ran.The Interaction Between Naringin and Human Serum Albumin[J].Henan Science,2014(5):716-718. |
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| Authors: | Wang Ling Zhao Junhong Yang Ran |
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| Institution: | 1. Department of Chemistry, Zhengzhou Normal University, Zhengzhou 450044, China： 2. Institute of Chemistry Co, Ltd., Henan Academy of Science, Zhengzhou 450002, China： 3. The College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450052, China) |
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| Abstract: | The interaction between naringin and Human Serum Albumin （HSA） was studied by fluorescence and synchronous fluorescence spectrum. The results suggested that the fluorescence of HSA was quenched by naringin and the interaction caused change of protein conformation. The quenching constant indicated that the quenching mechanism was a static quenching process. The distance was calculated to be 3.7 nm according to the theory of the Forster energy transference. |
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| Keywords: | HSA naringin fluorescence spectrum interaction mechanism |
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