20个荷花品种花粉活力测定及贮藏研究

为研究荷花花粉的生活力,选择了20个荷花品种分别用TTC染色法、亚甲基蓝染色法和离体萌发法测定了花粉的染色率和萌发率,并研究了不同温度贮藏条件对花粉生活力的影响.结果表明,亚甲基蓝染色法不能作为荷花花粉生活力的测定方法,离体萌发法结果更直观和准确;20个荷花品种间的离体萌发率差异明显,最高为13.1%,最低为0;不同品种在三种贮藏温度下萌发率变化趋势相同,4℃条件下贮藏较有利于荷花花粉生活力的保持.

Viability and Storage Capacity of Pollen among 20 Lotus Cultivars

To investigate the pollen viability of lotus,the pollen dyeing rate and germination rate of 20 lotus cultivars were determined by three testing methods including TTC coloration,methylene blue staining and culture in vitro. The effect of different storage temperature on the pollen vitality was also researched. The results indicated that the methylene blue staining is not suitable to be used for testing the lotus pollen viability and the results of culture in vitro are more accurate and visual than the others. There were significant differences in germination rate among the 20 lotus cultivars,the highest germination rate was 13.1% and the lowest was 0. The changing trends of germination rate were same under the three storage temperature among the different cultivars and the most proper temperature was 4℃for lotus pollen storage.