| 铜绿假单胞菌长链脂酰辅酶A合成酶的克隆及活性鉴定 |
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| 引用本文： | 王建林,邵新峰,梁振普,张小霞,谢斌. 铜绿假单胞菌长链脂酰辅酶A合成酶的克隆及活性鉴定[J]. 河南科学, 2014, 0(3): 355-361 |
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| 作者姓名： | 王建林 邵新峰 梁振普 张小霞 谢斌 |
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| 作者单位： | [1]河南农业大学生命科学学院,郑州450002 [2]瑞典隆德大学纯与应用生物化学系,隆德SE- 22100,瑞典 |
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| 基金项目： | 教育部新世纪优秀人才支持计划项目（NCET-10-0134） |
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| 摘 要： | 早期诊断对于II型糖尿病的预防和治疗尤为重要,游离脂肪酸是II型糖尿病的早期诊断的重要因素.铜绿假单胞菌的长链脂酰辅酶A合成酶(FadD1)是一种可以检测游离脂肪酸的酶.表达并纯化了FadD1,利用分光光度法对其活性进行了鉴定,鉴定结果显示FadD1对油酸钾有较好的生物学活性.为今后游离脂肪酸分析酶热传感器的研制奠定了基础.
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| 关 键 词： | 游离脂肪酸 长链脂酰辅酶A合成酶 原核表达 分光光度法 II型糖尿病早期诊断 |
Cloning and Activity Analysis of Long-chain Acyl-coenzyme A Synthetases from Pseudomonas aeruginosa |
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| Wang Jianlin,Shao Xinfeng,Liang Zhenpu,Zhang Xiaoxia,Xie Bin. Cloning and Activity Analysis of Long-chain Acyl-coenzyme A Synthetases from Pseudomonas aeruginosa[J]. Henan Science, 2014, 0(3): 355-361 |
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| Authors: | Wang Jianlin Shao Xinfeng Liang Zhenpu Zhang Xiaoxia Xie Bin |
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| Affiliation: | 1. College of Life Sciences, Henan Agricultural University, Zherlgzhou 450002, China; 2. Department of Pure and Applied Biochemistry, Lund University, Lund SE-22100, Sweden) |
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| Abstract: | Early identification of type II diabetes is important in prevention of this diseases. Free fatty acid has become an important factor in the early diagnosis of type II diabetes. In the current study, an acyl-coenzyme synthetase named FadD1 from Pseudomonas aeruginosa which can detect fi＇ee fatty acids has been expressed. The activity of FadD1 has been identified by spectrophotometry, and the result shows that it has a good effect to potassium oleate. It would be significant to develop a FFA enzyme thermal sensor. |
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| Keywords: | free fatty acids： long-chain fatty acyl--coenzyme A synthetase： prokaryotic expression： spectrophotometry：early identification of type II diabetes |
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