| Exendin-4抗噬菌体工程菌的选育 |
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| 引用本文： | 杨好冬,徐良,韦一昊,王小纯.Exendin-4抗噬菌体工程菌的选育[J].河南科学,2014(5):734-736. |
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| 作者姓名： | 杨好冬 徐良 韦一昊 王小纯 |
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| 作者单位： | 河南农业大学生命科学学院,郑州450002 |
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| 基金项目： | 河南省科技攻关项目（102101310600） |
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| 摘 要： | 利用自然突变法成功选育出了抗噬菌体的Exendin-4工程菌.该菌株抗性好,融合蛋白表达量高.但该菌株3次传代后菌种发生了衰退,稳定性下降,目的蛋白表达量下降,杂蛋白增多,已经不适合作为发酵菌株.而初代表达效果良好,保存菌种时应选用最先获得的初代菌株.
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| 关 键 词： | Exendin- 抗噬菌体 自然突变 稳定性 |
Screening for Anti-bacteriophage Engineering Strains of Exendin-4 |
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| Yang Haodong,Xu Liang,Wei Yihao,Wang Xiaochun.Screening for Anti-bacteriophage Engineering Strains of Exendin-4[J].Henan Science,2014(5):734-736. |
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| Authors: | Yang Haodong Xu Liang Wei Yihao Wang Xiaochun |
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| Institution: | (College of Life Science, Henan Agricuhural University, Zhengzhou 450002, China) |
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| Abstract: | In this study, the natural mutations were successfully used to breed anti-phage Exendin-4 engineering bacteria strains with good resistant and high expression of the fusion protein. After three passages, the strain bacteria occurred recession decreased stability, and decreased expression of the interest protein, increased other protein. It is not suitable as a fermentation strain. The first generation of strain obtained with good representatives, was the first choice for strain saving. |
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| Keywords: | Exendin-4 anti-bacteriophage natural mutations stability |
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