| DNS法测定纤维素酶活力最适条件研究 |
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| 引用本文： | 王琳. DNS法测定纤维素酶活力最适条件研究[J]. 河南师范大学学报(自然科学版), 1998, 26(3): 66-69 |
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| 作者姓名： | 王琳 |
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| 作者单位： | 河南师范大学生物系 |
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| 摘 要： | 鉴于纤维素酶研究和生产中，纤维素酶活力测定和酶活力单位的表示方法很不统一的现象，本文研究和分析了３，５－二硝基水杨酸法测定羧甲基纤维素酶糖化力的实验条件，如：温度、ｐＨ、酶促反应时间、ＤＮＳ显色时间、波长等．实验表明：在５０℃，ｐＨ４．６条件下酶促反应时间５ｍｉｎ，ＤＮＳ显色５ｍｉｎ，于４９０ｎｍ处测定ＯＤ值比较适宜．
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| 关 键 词： | 纤维素酶活力；3.5－二硝基水杨酸（DNS）；羧甲基纤维素（CMC） |
The Optimal Conditions for Cellulase Activity Measurement with DNS Method |
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| Wang Lin Liu Guosheng Wang Linsong Zhang Zhihong Hou Jinhuai Guo Huimin. The Optimal Conditions for Cellulase Activity Measurement with DNS Method[J]. Journal of Henan Normal University(Natural Science), 1998, 26(3): 66-69 |
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| Authors: | Wang Lin Liu Guosheng Wang Linsong Zhang Zhihong Hou Jinhuai Guo Huimin |
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| Abstract: | In the studying and producing of cellulase ,the method of cellulase activity measurement and enzyme activity expression is very diverse .So in this paper ,the conditions for carboxy-methy cellose(CMC)enzyme activity measurement was studied with 3,5-dinitrosalicylic acid(DNS)method ,The results show as follows :the appropriate enzyme reaction conditions are pH4.6,50℃ and 5 miniutes,the proper color-reaction is 5 miniutes and the optimal absorb wavelength is at 490nm. |
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| Keywords: | cellulase activity 3 5-dinotrosalicylic acid carboxymethylcellulose |
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