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| 黄金香柳的组织培养与快速繁殖 | |
| 引用本文： | 兰伟,杨京霞,王玲,王菲菲,郁琳洁. 黄金香柳的组织培养与快速繁殖[J]. 阜阳师范学院学报(自然科学版), 2013, 30(1): 36-39 |
| 作者姓名： | 兰伟 杨京霞 王玲 王菲菲 郁琳洁 |
| 作者单位： | 1. 阜阳师范学院生命科学学院,安徽阜阳,236037 2. 阜阳师范学院生命科学学院,安徽阜阳236037;抗衰老中草药安徽省工程技术研究中心 安徽阜阳236037 3. 安徽阜阳金丰生态农业有限公司,安徽阜阳,236001 |
| 基金项目： | 安徽省花木科技专家大院项目，阜阳师范学院自然科学重点项目，安徽省级科研平台委托专项课题 |
| 摘 要： | 以黄金香柳(Melaleuca bracteata cv.)为材料,研究基本培养基中不同浓度6-BA和NAA对黄金香柳试管苗增殖的影响。试验结果表明,温度为(25±1)℃、光照时间12 h·d-1、光照强度2000 LX的培养条件下,在13种培养基处理中,适宜黄金香柳试管苗快速增殖的培养基是MS+6-BA 0.1 mg·L-1+NAA 0.05 mg·L-1+琼脂6.2 g·L-1+蔗糖30 g·L-1,该培养基配比的每瓶试管苗平均增殖倍数可达16.17倍。丛生芽在1/2MS+琼脂6.2 g·L-1+蔗糖30 g·L-1的培养基上易生根,可以获得健壮完整的小苗。 |
| 关 键 词： | 黄金香柳 组织培养 快速繁殖 |
Tissue culture and rapid propagation of Melaleuca bracteata cv. |
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| LAN Wei , YANG Jing-xia , WANG Ling , WANG Fei-Fei , YU Lin-jie. Tissue culture and rapid propagation of Melaleuca bracteata cv.[J]. Journal of Fuyang Teachers College:Natural Science, 2013, 30(1): 36-39 | |
| Authors: | LAN Wei YANG Jing-xia WANG Ling WANG Fei-Fei YU Lin-jie |
| Affiliation: | 1. School of Life Sciences, Fuyang Teachers College, Fuyang Anhui 236037, China ;2. Anhui Provincial Engineering Technology Research Center of Anti-aging Chinese Herbal Medicine, Fuyang Anhui 236037, China; 3. Anhui Fuyang Jinfeng Ecological Agriculture Limited Company, Fuyang Anhui 236001, China) |
| Abstract: | Melaleuca bracteata cv, is used as test materials to study the influence of basal media with different concentrations of 6-BA and NAA on the proliferation of tube seedlings of Mlaleuca bracteata cv.. The experimental results show that under the culture condition that temperature is （25 ± 1 ）℃, illumination time is 12 h ·d-1, and light intensity is 2000LX, among the 13 culture media processing Melaleuca bracteata cv, the formula of the most suitable one for the rapid proliferation of the tube seedlings is MS ＋ 6-BA0.1 mg·L-1 ＋ NAAof0.05 mg·L-1 ＋ AGAR6.2 g·L-1 ＋ sucrose 30g·L-1. The average proliferation rate of the seedlings in every tube proportioned by the culture medium can reach 16.17. In addition, the cluster buds root easily in I/2MS ＋ AGAR 6.2 g·L-1 ＋ sucrose 30 g ·L-1 culture medium, and the robust plants of Melaleuca bracteata cv. are also obtained. |
| Keywords: | Melaleuca bracteata cv. tissue culture rapid propagation |
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