| 三元全酶系统的共固定化和辅酶再生 |
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| 引用本文： | 徐俊,楼一心,朱正华,赵成.三元全酶系统的共固定化和辅酶再生[J].华东理工大学学报(自然科学版),1989(4). |
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| 作者姓名： | 徐俊 楼一心 朱正华 赵成 |
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| 作者单位： | 华东化工学院生化工程研究所
(徐俊，楼一心，朱正华)，华东化工学院生化工程研究所(赵成) |
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| 摘 要： | 本文研究了用对-氨基苯砜乙基(ABSE)-交联琼脂糖做载体,通过戊二醛共价交联,对醇脱氨酶、乳酸脱氢酶、辅酶Ⅰ进行了位置对位置的共固定化。按本实验方法得到的固定化三元全酶系统的辅酶再生能力为相应游离酶系统的5倍。本文还对该三元全酶系统的固定化机理和辅酶再生性能做了一定的讨论。
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| 关 键 词： | 固相化 烟酰胺腺嘌呤二核苷酸 醇脱氢酶 乳酸脱氢酶 共固定化 辅酶再生 戊二醛 |
Site-to-Site Coimmobilization of Dehydrogenases and NAD for NAD Regeneration |
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| Xu Jun,Lou Yixin,Zhu Zhenghua Zhao Cheng.Site-to-Site Coimmobilization of Dehydrogenases and NAD for NAD Regeneration[J].Journal of East China University of Science and Technology,1989(4). |
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| Authors: | Xu Jun Lou Yixin Zhu Zhenghua Zhao Cheng |
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| Institution: | Research Institute of Biochemical Engineering |
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| Abstract: | Alcohol dehydrogenase, lactate dehydrogenase and NAD were immobilized separately and coimmobilized site-to-site on p-aminobenzenesulfonylethyl-CL sepharose activated previously with glutaraldehyde. The regeneration ability of NAD with the coimmobilized tribasic enzyme system obtained here was five times as high as that with the corresponding free enzyme system. And finally, the coimmobilization process and NAD regeneration ability of the tribasic enzyme system were discussed. |
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| Keywords: | immobilization coenzyme I alcohol dehydrogenase lactate dehydrogenase coenzyme regeneration glutaraldehyde |
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