| 盐胁迫下沙枣组织再生芽的分化和生长 |
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| 引用本文： | 张建民,姚敦义.盐胁迫下沙枣组织再生芽的分化和生长[J].曲阜师范大学学报,1992,18(3):65-68. |
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| 作者姓名： | 张建民 姚敦义 |
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| 作者单位： | 曲阜师范大学生物系，山东师范大学生物系 273165，山东曲阜市，250014，山东济南市 |
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| 摘 要： | 沙枣的各种外植体在 B_5+0.2mg/l IBA+3mg/l KT 的培养基上进行组织培养;20天后可分化出大量再生芽,待长至0.3～0.5cm 时,转入 MS 培养基上,可形成完整的再生植株.在试验时,把再生芽分别转入含0、0.3、0.5、0.8、1.0(%)NaCl 的 B_5培养基中培养,发现在0.3%NaCl 下生长分化良好.随着 NaCl 含量的提高,再生芽分化率、生长率均下降。
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| 关 键 词： | 沙枣 外植体 氯化钠 组织培养 |
RESEARCHES ON TISSUE CULTURE AND SALT-RESISTANCE OF NARROW-LEAVED OLEASTER |
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| Zhang Jianmin.RESEARCHES ON TISSUE CULTURE AND SALT-RESISTANCE OF NARROW-LEAVED OLEASTER[J].Journal of Qufu Normal University(Natural Science),1992,18(3):65-68. |
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| Authors: | Zhang Jianmin |
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| Institution: | Zhang Jianmin Department of Biology,Qufu Normal University,273165 Qufu,Shandong,PRCYao Dunyi Department of Biology,Shandong Normal University,250014,Jinan,Shandong,PRC |
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| Abstract: | Various explant of Narrow-leaved oleaster can grow and differen-tiate rapidly on B_5 medium including hormone.It produce a lot of leaf buds in20 days.Then these leaf buds are transfered and cultured continually in medi-um containing salt.They grow well in low salt-content,but grow and differ-entiate badly in high salt-content. |
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| Keywords: | Narrow-leaved oleaster explant NaCl tissue culture |
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