Identification of cancer stem cell-like cells from human epithelial ovarian carcinoma cell line |
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| Authors: | M F Shi J Jiao W G Lu F Ye D Ma Q G Dong X Xie |
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| Institution: | (1) Department of Gynecologic Oncology, Women’s Hospital, School of Medicine, Women’s Reproductive Health Laboratory of Zhejiang Province, Zhejiang University, 2 Xueshi Road, Hangzhou, Zhejiang, China;(2) Shanghai Cancer Institute, Shanghai Jiao Tong University, 2200 Xietu Road, Shanghai, China;(3) Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China; |
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| Abstract: | Cancer stem cells (CSCs) play an important role in the development, invasion, and drug resistance of carcinoma, but the exact
phenotype and characteristics of ovarian CSCs are still disputable. In this study, we identified cancer stem cell-like cells
(CSC-LCs) and investigated their characteristics from the ovarian adenocarcinoma cell line 3AO. Our results showed that CSC-LCs
were enriched in sphere-forming test and highly expressed CD44+CD24−. The spheres and CD24− cells possessed strong tumorigenic ability by transplantation into nonobese diabetic/severe combined immunodeficient mice.
CD44+CD24− cells expressed stem cell markers and differentiated to CD44+CD24+ cells by immunofluorescence assay and fluorescence-activated cell-sorting analysis. In vitro experiments verified that CD44+CD24− cells were markedly resistant to carboplatin and paclitaxol. In conclusion, our study identifies the CD44+CD24− phenotype, self-renewal, high tumorigenicity, differentiation potential, and drug resistance of ovarian CSC-LCs. Our findings
may provide the evidence needed to explore a new strategy in the treatment of ovarian cancer. |
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