响应面法优化正红菇菌丝体产胞内凝集素培养条件

采用响应面法对液体深层培养的正红菇(Russula vinosa)菌丝体产胞内凝集素的培养条件进行了优化.通过单因子实验确定最适碳源和氮源为淀粉和酵母膏,在此基础上,通过Plackett-Burrman设计对影响其产胞内凝集素的相关因素进行评估并筛选出具有显著效应的淀粉、酵母膏、装液量3个因素,通过最陡爬坡实验及响应面分析法确定了产凝集素的最适条件:ω(淀粉)3.04%,ω(酵母膏)0.3%,ω(KH2PO4)0.2%,ω(MgSO4)0.1%,装液量73mL,转速250r/min,初始pH5,发酵时间5d.在此条件下,菌丝体产胞内凝集素的总活力可达72127.3u,与初始培养条件下的凝集素总活力相比提高了1.88倍.

Optimization of Intracellular Lectin Production for Russula vinosa Mycelium by Response Surface Methodology

Russula vinosa fermentation recipe was optimized by surface response methodology to improve production of lectin from mycelium of Russula vinosa. The optimum carbon and nitrogen for Russula vinosa mycelium were obtained through monofactorial experiment, which were starch and yeast extract respectively. Through Plackett-Burrman design some process factors of intracellular lectin production were evaluated and three factors had prominent effect on intracellular lectin production were picked out, which were starch, yeast extract and liquid volume respectively. The steepest ascent search and response surface analysis were used to estimate optimum culture condition. It was composed of 3.04% starch, 0.3% yeast extract, 0.2% KH_2PO_4, 0.1% MgSO_4,liquid volume 73 mL,250 r/min,pH 5,fermentation time 5 d. As a result , the total agglutinating activity of intracellular lectin was increased to 72 127.3 u, which was improved 1.88-fold compared to the original conditions.