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Appearance of sperm activation factors in the ovary of the sea urchinHemicentrotus pulcherrimus with maturation
Authors:N Suzuki  K Kobayashi  S Isaka
Institution:(1) Department of Biochemistry, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, 173 Tokyo, (Japan);(2) Present address: Department of Biology, College of General Education, University of Chiba, 1-33 Yayoi-cho, 280 Chiba, (Japan);(3) Present address: Noto Marine Laboratory, Kanazawa University, Ogi, Uchiura, 927-05 Ishikawa, (Japan)
Abstract:Summary Ovaries of the sea urchinHemicentrotus pulcherrimus were extracted with 70% ethanol. The extracts were polarographically assayed for respiration-stimulating activity toward homologous spermatozoa. The data of the bioassay and light microscopic observation on thin sections of the ovaries showed detectable activity only in mature ovaries.We thank the Misaki Marine Biological Station for kindly supplying us with specimens. This work was supported in part by a grant from the Ministry of Education, Science and Culture of Japan.
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