壳聚糖基蛋白质分子印迹聚合物的制备及识别性能研究

通过对壳聚糖化学改性,引入羧甲基和丙烯酸酯功能基,合成水溶性、可聚合和可交联的壳聚糖衍生物.FTIR分析结果表明,甲基丙烯酸缩水甘油酯(GMA)通过环氧基团开环反应与羧甲基壳聚糖(CM-CS)的羟基形成醚键键联,生成的壳聚糖衍生物(CMCS-GMA)可在过硫酸铵作用下发生聚合反应.采用表面接枝的方法在聚苯乙烯微孔板上形成CMCS-GMA的聚合物薄层,并用于在水相中印迹血红蛋白分子.蛋白质实验的结果表明,印迹聚合物结合血红蛋白的能力明显高于非印迹聚合物.进一步考察该印迹聚合物对血红蛋白的结构相似物(溶菌酶)的结合特性,显示了较高的识别选择性.

Preparation of Chitosan Derivative Based Molecular Imprinted Polymer and Its Recognition for Protein

Chitosan derivative with aqueous solubility and containing polymerisable and crosslinkable functional groups were synthesized through chemical modification using chloroacetic acid and glycidyl methacrylate (GMA). FTIR analysis showed that GMA reacted with carboxymethyl chitosan(CMCS)by a ring opening reaction and the resultant(CMCS-GMA)could polymerize using ammonium persulphate as initiator. Thin films of CMCS-GMA polymer were formed on the surface of polystyrene microplates and were applied to imprint haemoglobin (Hb) in aqueous medium. The imprinted chitosan derivative has much higher binding capacity for Hb than the non-imprinted polymer with the same chemical composition. Protein assay of lysozyme, a structural analogue of haemoglobin, showed that the imprinted polymer also exhibits a higher selectivity for the imprinted molecule.