| 人16型乳头瘤病毒"假病毒"的制备 |
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| 引用本文： | 秦焱,崔红莲,胡美浩. 人16型乳头瘤病毒"假病毒"的制备[J]. 北京大学学报(自然科学版), 2006, 42(2): 180-183 |
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| 作者姓名： | 秦焱 崔红莲 胡美浩 |
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| 作者单位： | 北京大学生命科学学院,北京,100871;北京大学生命科学学院,北京,100871;北京大学生命科学学院,北京,100871 |
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| 摘 要： | 利用杆状病毒表达体系,在sf9细胞中表达人16型乳头瘤病毒(Human Papillomavirus type 16, HPV16)的晚期表达蛋白L1,通过超速离心的方法得到HPV16病毒样颗粒(virus like particles, VLPs).VLPs经过DTT和EDTA处理解聚成L1蛋白,将构建好的真核表达载体E6E7-pcDNA3.1 与解聚的L1蛋白混合,透析除去DTT和EDTA并逐渐升高Ca2 的浓度,解聚的L1能够重新聚集成VLPs,其中一部分VLPs包裹了真核表达载体,和天然的人乳头瘤病毒的构成很相似,作者称之为"假病毒".
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| 关 键 词： | 人16型乳头瘤病毒 杆状病毒表达系统 假病毒 |
| 收稿时间： | 2005-06-17 |
| 修稿时间： | 2005-06-172005-12-13 |
In Vitro Construction of Pseudovirions of Human Papillomavirus Type 16 |
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| QIN Yan,CUI Honglian,HU Meihao. In Vitro Construction of Pseudovirions of Human Papillomavirus Type 16[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2006, 42(2): 180-183 |
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| Authors: | QIN Yan CUI Honglian HU Meihao |
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| Affiliation: | College of life sciences, Peking University, Beijing, 100871 |
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| Abstract: | The L1 major capsid protein of human papillomavirus type 16 was expressed in sf9 insect cells by recombinant baculoviruses and self-assembled into virus-like particles (VLPs). The VLPs were harvested by CsCl ultracentrifugation and were disassembled with DTT and EDTA. A reassembled process was performed by removing DTT and EDTA in the presence of E6E7-pcDNA3.1 plasmid and Ca 2 . The novel VLPs, in which the plasmid was packed, were named HPV16 pseudovirus. |
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| Keywords: | human papillomavlrus type 16 baculovirus expression system pseudovirus |
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