High expression of a rice homeobox inE. coli |
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Authors: | Cheng Xing-guo Chen Nai-qing Yi Qing-ming |
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Affiliation: | (1) College of Life Sciences, Wuhan University, 430072 Wuhan, China |
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Abstract: | Homeotic genes share a characteristic DNA segment, the homeobox, which encodes a defined domain of the homeotic protein-homeodomain which seems to mediate the binding to specific DNA sequences, whereby the homeotic protein exerts a gene regulatory function. In this study, the homeodomain encoded by the OSIH-1 (designated fromOryza sativa-Indica homeobox-1) homeobox of rice was overproduced in a expression vector inE. coli, as a form of inclusion body and analyzed by Western blotting and crossed-immunoreaction. Crossed-immunoreaction studies among OSIH-1, Kn-1 and Quox-1 indicate all three are homologous at the protein level. The OSIH-1 homeodomain is then to identify the homeotic target genes. Foundation item: Supported by the National Natural Science Foundation of China (#39570370) Biography: CHENG Xing-guo (1973-), male, Graduate student. |
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Keywords: | rice homeobox homeodomain gene expression |
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