| 单克隆抗体亲和层析最适洗脱液筛选研究 |
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| 引用本文： | 阎静辉,邹岳奇.单克隆抗体亲和层析最适洗脱液筛选研究[J].河北省科学院学报,1991(4):47-51. |
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| 作者姓名： | 阎静辉 邹岳奇 |
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| 作者单位： | 河北省科学院生物研究所，河北省科学院生物研究所，河北省科学院生物研究所 |
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| 摘 要： | 本文介绍一种筛选单克隆抗体(McAb)亲和层析最适洗脱液的方法,一种经过改进的双抗体夹心酶联免疫吸附检测法(DAS—ELISA),其中以联有一种McAb的经CNBr活化的Sepharose凝胶为固相,另一种McAb标上辣根过氧化物酶作为标记抗体。我们在以单抗亲和层析纯化促卵泡激素(FSH)的工作中使用该方法进行了最适洗脱液的筛选,取得了很好的结果。该方法简便、快速、可靠。
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| 关 键 词： | 单克隆抗体 免疫亲和层析 促卵泡激素 洗脱液 酶免疫测定法 |
RESEARCH ON SCREENING THE OPTIMAL ELUENT IN MONOCLONAL ANTIBODY AFFINITY CHROMATOGRAPHY |
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| Yan Jinghui Zou Yueqi Liu Yucui.RESEARCH ON SCREENING THE OPTIMAL ELUENT IN MONOCLONAL ANTIBODY AFFINITY CHROMATOGRAPHY[J].Journal of The Hebei Academy of Sciences,1991(4):47-51. |
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| Authors: | Yan Jinghui Zou Yueqi Liu Yucui |
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| Institution: | Yan Jinghui Zou Yueqi Liu Yucui |
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| Abstract: | An improved method of screening the optimal eluent in monoclonal antibody (McAb) affinity chromatography is developed and recommended. It is a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), in which the solid phase is a CNBr-activated Sepharose 4B gel linked with a McAb and another McAb labeled with horseradish peroxidase (HRP)is used as the marker antibody. The method has been applied to screen an optimal eluent in an affinity chromatography of purifying a kind of follicle-stimulating hormone (FSH)and obtained excellent results. The method is simple, rapid and reliable. |
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