Differential expression of glucose transporters during chick embryogenesis |
| |
Authors: | FM Carver IA Shibley Jr JS Pennington SN Pennington |
| |
Institution: | (1) Department of Biochemistry, Brody School of Medicine, East Carolina University, Greenville, (North Carolina 27858, USA), Fax +1 252 816 3383, e-mail: carverf@mail.ecu.edu , US;(2) Department of Comparative Medicine, Brody School of Medicine, East Carolina University, Greenville, (North Carolina 27858, USA), US |
| |
Abstract: | The patterns of Glut1 and Glut3 glucose transporter protein and mRNA expression were assessed during embryogenesis of chicken
brain and skeletal muscle, Glut4 protein levels were also evaluated in skeletal muscle and heart, and Glut1 was examined in
the developing heart and liver. Glut1 protein expression was detectable throughout brain ontogeny but was highest during early
development. Glut1 mRNA levels in the brain remained very high throughout development. Glut3 protein was highest very early
and very late and mRNA was highest during the last half of development. In embryonic skeletal muscle, the levels of Glut1and
Glut3 proteins and mRNA were highest very early, and declined severely by mid-development. Glut1 protein and mRNA in the heart
also peaked early and then decreased steadily. Although Glut1 mRNA levels were consistently high in the embryonic liver, Glut1
protein expression was not detected. These results suggest that (1) Glut1 is developmentally regulated in chick brain, skeletal
muscle, and heart, (2) Glut1 mRNA is present in liver but does not appear to be translated, (3) Glut3 in brain increases developmentally
but is virtually absent in muscle, and (4) Glut4 protein and mRNA appear to be absent from chick heart and skeletal muscle.
Received 11 January 2001; accepted 14 February 2001 |
| |
Keywords: | , Development, chick, Glut1, Glut3, Glut4, embryogenesis, |
本文献已被 PubMed SpringerLink 等数据库收录! |
|