ERK activation by Ca2+ ionophores depends on Ca2+ entry in lymphocytes but not in platelets, and does not conduct membrane scrambling |
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Authors: | A Arachiche I Badirou J Dachary-Prigent I Garcin D Geldwerth-Feniger D Kerbiriou-Nabias |
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Institution: | (1) INSERM U770 and Université Paris-Sud, 80 rue du Général Leclerc, 94276 Le Kremlin-Bicêtre cedex, France;(2) INSERM U688 and Université Victor Segalen, Bordeaux, France;(3) INSERM UMR-S757 and Université Paris-Sud, Orsay, France |
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Abstract: | Rapid Ca2+-dependent phospholipid (PL) reorganization (scrambling) at the plasma membrane is a mechanism common to hematopoietic cells
exposing procoagulant phosphatidylserine (PS). The aim of this research was to determine whether activation of the extracellular
signal-regulated kinase (ERK) pathway was required for PL scrambling, based on a single report analyzing both responses induced
by Ca2+ ionophores in megakaryoblastic HEL cells. Ca2+ ionophore-stimulated ERK phosphorylation was induced in platelets without external Ca2+, whereas exogenous Ca2+ entry was crucial for ERK activation in Jurkat T cells. In both cells, membrane scrambling only occurred following Ca2+ entry and was not blocked by inhibiting ERK phosphorylation. Furthermore, ERK proteins are strongly phosphorylated in transformed
B lymphoblastic cell lines, which do not expose PS in their resting state. Overall, the data demonstrated that ERK activation
and membrane scrambling are independent mechanisms.
A. Arachiche, I. Badirou: These authors contributed equally to this work.
Received 18 June 2008; received after revision 24 September 2008; accepted 1 October 2008 |
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Keywords: | " target="_blank"> Extracellular signal-regulated kinase phosphatidylserine phospholipid scrambling platelet Jurkat B lymphocytes Scott syndrome |
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