小鼠睾丸发育全过程的组织学观察

为系统观察小鼠睾丸组织发育过程,将生后1～57d处于不同发育时期的17组昆明种正常小鼠睾丸组织制备石蜡切片、进行H.E染色分析。结果表明:小鼠生后初期睾丸曲细精管中只有支持细胞和原始生精细胞;至生后8d时出现B型精原细胞;15d时出现初级精母细胞;23d时出现次级精母细胞及少量圆形精子细胞,此后圆形精子细胞逐渐增多;30d时圆形精子细胞发生变态;36d时大量精子开始稳定出现于曲细精管管腔中,并延续至此后各期。这意味着小鼠生精细胞在出生后经过一个相对连续的发育分化过程,至生后约36d发育成熟。这为细化小鼠睾丸组织发育和精子发生过程提供了详细资料。

The Histological Observation in the Testis of Mice at Different Development Stage

To observe the development of testis in mice,the testis of total 17 groups of Kunming normal mice at their different developmental stages among 1 ～ 57 days after birth was examed by using the paraffin tissue section and HE staining. The result showed that only sertoli cells and primitive germ cells exhibited in seminiferous tubules of the early postnatal mice testis; B-type spermatogonia were observed at the 8th day after birth; Primary spermatocytes were observed at the 15th day; Secondary spermatocyte and little round spermatids appeared at the 23th day and then the quantity of round spermatids were gradually increased. At the 30th day,long-shaped sperm cells exhibited. At the 36th day,a large sum of sperms were observed in seminiferous tubules and lasted to whole subsequent stages. It means that via a process of continuously development and differentiation the primitive germ cells matured in about 36 days after birth. This study revealed more details on the development of mice testis and sperms.