| 酶催化动力学光度法测定铜(Ⅱ) |
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| 引用本文： | 柳畅先,朱苗苗,王艺.酶催化动力学光度法测定铜(Ⅱ)[J].中南民族大学学报(自然科学版),2014(3):24-26. |
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| 作者姓名： | 柳畅先 朱苗苗 王艺 |
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| 作者单位： | 中南民族大学化学与材料科学学院,武汉,430074 |
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| 基金项目： | 中南民族大学自然科学基金资助项目 |
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| 摘 要： | 基于脲酶(UASE)催化尿素水解的反应中Cu2+对于酶的抑制作用原理,通过测定产物吸光度的变化率得出了其酶促反应速度,对应于Cu2+浓度而制得标准曲线,检出限为0.05μmol/L.讨论了pH值和温度对测定的影响,结果表明:该法快速灵敏.
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| 关 键 词： | 铜(Ⅱ) 测定 动力学光度法 |
Enzymatic Spectrophotometric Determination of Copper(II) |
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| Liu Changxian,Zhu Miaomiao,Wang Yi.Enzymatic Spectrophotometric Determination of Copper(II)[J].Journal of South-Central Univ for,2014(3):24-26. |
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| Authors: | Liu Changxian Zhu Miaomiao Wang Yi |
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| Institution: | (College of Chemistry and Material Science, South-Central University for Nationalities, Wuhan 430074, China) |
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| Abstract: | A spectroscopic method was developed to determine copper （Ⅱ） content based on the inhibition of copper （Ⅱ） for the hydrolysis of urea catalyzed by urease （ UASE） .The rate of the absorbance change of the product was measured for the calculation of the rate of the enzymatic reaction and a standard curve of copper （Ⅱ） was prepared .The detection limit could reach 0.05μmol/L, and the effects of the pH value and temperature were also discussed .The results indicated that the method was rapid and sensitive . |
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| Keywords: | Cu(Ⅱ) determination kinetic spectrophotometry |
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