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紫锥菊的组织培养及其实用快速繁殖
引用本文:王伯初,刘万钱,段传人. 紫锥菊的组织培养及其实用快速繁殖[J]. 重庆大学学报(自然科学版), 2005, 28(5): 120-122
作者姓名:王伯初  刘万钱  段传人
作者单位:重庆邮电学院,生物信息学院,重庆,400065;重庆大学,教育部生物力学和组织工程重点实验室,重庆,400030;重庆大学,教育部生物力学和组织工程重点实验室,重庆,400030
基金项目:重庆邮电学院校科研和教改项目
摘    要:利用植物组织培养技术快速育苗是一个新兴的生物技术应用领域.文中采用组织培养技术,以MS培养基为基本培养基,通过调节植物生长激素的配比对紫锥菊组织进行愈伤化、继代、分化、生根等系列培养,培育出完整植株,并采用声波刺激方式炼苗以提高移栽成活率.该方法避免了传统紫锥菊育苗时种子采集困难,发芽率低,难以大规模育苗的特点,从而为建立规模化培育紫锥菊苗以及为我国开发利用紫锥菊药用资源提供了理论依据.

关 键 词:紫锥菊  组织培养  育苗  声波刺激
文章编号:1000-582X(2005)05-0120-03
修稿时间:2004-12-17

Tissue Culture and Rapid Propagation of Echinacea Purpurea
WANG Bo-chu,LIU Wan-qian,DUAN Chuan-ren. Tissue Culture and Rapid Propagation of Echinacea Purpurea[J]. Journal of Chongqing University(Natural Science Edition), 2005, 28(5): 120-122
Authors:WANG Bo-chu  LIU Wan-qian  DUAN Chuan-ren
Abstract:It is a burgeoning application field for the biotechnology that to apply the technique of plant tissue culture breed seedling. The authors study the responses of Echinacea purpurea to different proportion plant growth hormone or auxin through adopting the culture tissue technique on the MS. The integrated seedling of Echinacea purpurea can be cultivated by callus, subculture, differentiation and rhizogenesis. The surviving rate of Echinacea purpurea seedling transplant are improved by appropriate sound stimulation appropriate. Study harvest offers the gist for the large-scale breeding seedling and the officinal resource exploitation of Echinacea purpurea in our country, and avoid some traditional handicap in breeding seedling of Echinacea purpurea, such as the difficult of seed collection, low burgeoning rate, and the disadvantage for large-scale propagation.
Keywords:echinacea purpurea  tissue culture  breed seedling  sound stimulation
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