| DNA与蛋白质结合的共振光散射研究及应用 |
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| 引用本文： | 李艳坤.DNA与蛋白质结合的共振光散射研究及应用[J].河北大学学报(自然科学版),2011,31(5):502-507. |
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| 作者姓名： | 李艳坤 |
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| 作者单位： | 华北电力大学环境科学与工程学院,河北保定,071003 |
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| 基金项目： | 河北省自然科学基金资助项目(B2011502061); 中央高校基本科研业务费专项资金项目(09QL52) |
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| 摘 要： | 在pH 2.21～4.35的Britton-Robinson(BR)缓冲溶液中,脱氧核糖核酸(DNA)与蛋白质相互作用形成复合物,引起较DNA或蛋白质单独存在时的共振光散射(RLS)信号的强烈增强.用于分析蛋白质与DNA结合的相互作用.实验结果显示:DNA与蛋白质之间存在较强的静电相互作用.在最佳实验条件下,波长315...
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| 关 键 词： | 共振光散射 蛋白质 脱氧核糖核酸 相互作用 |
Study on the Combination of DNA with Protein by Resonance Light Scattering Technique and Its Analytical Application |
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| LI Yan-kun.Study on the Combination of DNA with Protein by Resonance Light Scattering Technique and Its Analytical Application[J].Journal of Hebei University (Natural Science Edition),2011,31(5):502-507. |
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| Authors: | LI Yan-kun |
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| Institution: | LI Yan-kun(College of Environment Science and Engineering,North China Electric Power University,Baoding 071003,China) |
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| Abstract: | Proteins bind DNA to form a complex,which is bigger than DNA or proteins,leading to strong enhancement of resonance light scattering(RLS) intensity.Based on this,the complex of proteins with DNA results in strong enhancement of resonance light scattering(RLS) intensity in a Britton-Robinson(BR) buffer(pH 2.21-4.35).The experimental results showed the strong electrostatic binding force between proteins and DNA.Under the optical conditions,the enhanced RLS intensity at 315.0 nm was proportional to the concent... |
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| Keywords: | resonance light scattering proteins deoxyribonucleic acid interaction |
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