| Cloning and Expression of BTB/POZ-Domain from Zebrafish gcl （Germ Cell-Less）, and Appraisement of Its Polyclonal-Antibody |
| |
| 作者姓名： | DENG Fengjiao CHENG Pengxiang LAN Qiang XU Yan ZHUANG Tiangang |
| |
| 作者单位： | [1]College of Life Sciences, Wuhan University / Key Laboratory of the Ministry of Education for Developmental Biology, Wuhan 430072, Hubei, China [2]Center for Research on Reproduction and Women＇s Health, University of Pennsylvania Medical Center, Philadelphia, PA 19104-6080, USA |
| |
| 基金项目： | Supported by the National Natural Science Foundation of China （30570968, 30370744） |
| |
| 摘 要： | In this study, a recombinant pET28c-gBTB/POZ was constructed by cloning the sequence of the BTB/POZ domain of the zebrafish gcl （germ cell-less） into the expression vector pET28c, and pET28c-gBTB/POZ was transformed into BL21（DE3） pLysS strain to express the fusion protein for the preparation of antibody. Polyclonal-antibody against the GCL-BTB/POZ domain was prepared by immunizing rabbit with the fusion protein, and the Western Blot and immuno-histochemical analysis were performed to detect the quantity of the polyclonal-antibody. The result indicates that the polyclonal-antibodies were of good quantity and specification. Further studies will be performed to demonstrate the function and expression pattern of the GCL protein during the development process of zebrafish with the polyclonal-antibody.
|
| 关 键 词： | 发生遗传学 克隆技术 生物化学 多克隆技术 |
| 文章编号： | 1007-1202（2008）01-0123-06 |
| 收稿时间： | 2007-03-11 |
Cloning and expression of BTB/POZ-domain from zebrafish <Emphasis Type="Italic">gcl</Emphasis> (germ cell-less), and appraisement of its polyclonal-antibody |
| |
| DENG Fengjiao CHENG Pengxiang LAN Qiang XU Yan ZHUANG Tiangang.Cloning and expression of BTB/POZ-domain from zebrafish gcl (germ cell-less), and appraisement of its polyclonal-antibody[J].Wuhan University Journal of Natural Sciences,2008,13(1):123-128. |
| |
| Authors: | Fengjiao Deng Pengxiang Cheng Qiang Lan Yan Xu Tiangang Zhuang |
| |
| Institution: | (1) College of Life Sciences, Wuhan University / Key Laboratory of the Ministry of Education for Developmental Biology, Wuhan, 430072, Hubei, China;(2) Center for Research on Reproduction and Women’s Health, University of Pennsylvania Medical Center, Philadelphia, PA 19104-6080, USA |
| |
| Abstract: | In this study, a recombinant pET28c-gBTB/POZ was constructed by cloning the sequence of the BTB/POZ domain of the zebrafish
gcl (germ cell-less) into the expression vector pET28c, and pET28c-gBTB/POZ was transformed into BL21(DE3) pLysS strain to express
the fusion protein for the preparation of antibody. Polyclonal-antibody against the GCL-BTB/POZ domain was prepared by immunizing
rabbit with the fusion protein, and the Western Blot and immuno-histochemical analysis were performed to detect the quantity
of the polyclonal-antibody. The result indicates that the polyclonal-antibodies were of good quantity and specification. Further
studies will be performed to demonstrate the function and expression pattern of the GCL protein during the development process
of zebrafish with the polyclonal-antibody.
Biography: DENG Fengjiao(1954–), female, Professor, research direction: developmental biology. |
| |
| Keywords: | gcl (germ cell-less) polycolonal-antibody BTB/POZ domain zebrafish (Danio rerio) |
| 本文献已被 维普 SpringerLink 等数据库收录！ |
