| HPLC法测定金不换各部位中大黄酸、大黄素含量 |
| |
| 引用本文： | 丁艳霞,吴宏欣,李勉,李亚真.HPLC法测定金不换各部位中大黄酸、大黄素含量[J].云南民族大学学报(自然科学版),2012,21(1):6-9. |
| |
| 作者姓名： | 丁艳霞 吴宏欣 李勉 李亚真 |
| |
| 作者单位： | 1. 河南大学天然药物研究所,河南开封,475001
2. 河南大学校医院,河南开封,475000 |
| |
| 基金项目： | 河南省教育厅自然科学基金(2008A360003) |
| |
| 摘 要： | 采用高效液相色谱法测定河南栽培金不换中大黄酸、大黄素的含量,色谱柱为Hy-persilC18柱(250mm×4.6mm×5μm),流动相:甲醇∶0.25%磷酸(体积比80∶20),流速:1.1mL/min,检测波长为436nm.结果:大黄酸在0.154～1.54μg/mL、大黄素在0.412～2.06μg/mL范围内其峰面积积分值与质量浓度之间呈良好的线性关系(r=0.999 6和0.999 9),平均回收率分别为100.2%、99.87%(n=6),RSD分别为1.47%、2.1%.该方法简便、准确,可用于金不换中大黄酸、大黄素含量的测定.
|
| 关 键 词： | 金不换 大黄酸 大黄素 高效液相 |
Determination of Rhein and Emodin in Rumex obtusifolius L.by HPLC |
| |
| DING Yan-xia
,
WU Hong-xin
,
LI Mian
,
LI Ya-zhen.Determination of Rhein and Emodin in Rumex obtusifolius L.by HPLC[J].Journal of Yunnan Nationalities University:Natural Sciences Edition,2012,21(1):6-9. |
| |
| Authors: | DING Yan-xia WU Hong-xin LI Mian LI Ya-zhen |
| |
| Institution: | 1 (1.Institute of Natural Products,Henan University,Kaifeng 475001,China;2.Hospital of Henan University,Henan University,Kaifeng 475001,China) |
| |
| Abstract: | The research establishes a HPLC method for the determination of Rhein and Emodin in Rumex obtusifolius L grown in Henan.Hypersil C18(250 mm×4.6mm,5μm)and mobile phase methanol-phosphoric acid(80∶20) were used.The flow rate was 1.1 mL/min and the UV detection was 436 nm.The result showed that the linear range of Rhein concentration was from 0.154 μg/mL to 1.54 μg/mL(r=0.9996),while Emodin was between 0.412-2.06μg/mL,and its linear relation was good(r=0.9996 and 0.9999).The average recovery rate was 100.2%,and 99.87%(n=6) with the RSD of 1.47% and 2.1%.The method is simple,accurate,reproducible and applicable to the determination of the content of Rhein and Emodin in Rumex obtusifolius L.. |
| |
| Keywords: | Rumex obtusifolius L Rhein Emodin HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
