| 具有自主复制功能的水稻高度重复序列ARS2的结构和功能分析 |
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| 引用本文： | 程钢.具有自主复制功能的水稻高度重复序列ARS2的结构和功能分析[J].复旦学报(自然科学版),1998,37(4):469-476. |
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| 作者姓名： | 程钢 |
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| 作者单位： | 复旦大学遗传学研究所 |
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| 摘 要： | 对一个水稻珍汕９７Ａ不育系核ＤＮＡ来源的具有自主复制功能的高度重复顺序片段ＡＲＳ２（全长４７２０ｂｐ）进行了亚克隆构建和测序，获得了它的全顺序。
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| 关 键 词： | 高度重复序列 自主复制序列 水稻 微卫生DNA |
DNA Sequencing and Functional Analysis of Rice ARS2 |
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| Cheng Gang, Yang Kaiyong, Ao Wanyuan, Ren Daming.DNA Sequencing and Functional Analysis of Rice ARS2[J].Journal of Fudan University(Natural Science),1998,37(4):469-476. |
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| Authors: | Cheng Gang Yang Kaiyong Ao Wanyuan Ren Daming |
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| Abstract: | Subcloning and sequencing have been performed on a highly repetitive fragmentwith autonomously replicating capability from rice Zhenshan 97A nucleic DNA, whose lengthis 4 270 bp. The DNA segments were sequenced. It has high AT ratio (69%), two ARS core sequences(A/TTTTAPuTTTA/T) and 7 similar ones which comes with ARS characteristic sequence TNTPuAA at its upstream and/or downstream. Two key regions of ARS function are delimited in it with length of 400 bp and 360 bp. A microsatellite of rice locus genome(AT)n with high polymorphism was observed for the first time. |
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| Keywords: | highly repeated sequences autonomously replicating sequences microsatellite DNA |
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