Mass spectrometry for protein and peptide characterisation |
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Authors: | Jonsson A P |
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Institution: | (1) Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm (Sweden), Fax: +46 8 33 74 62, e-mail: Andreas.Jonsson@mbb.ki.se, SE |
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Abstract: | Mass spectrometry has become an important analytical tool in biological and biochemical research. Its speed, accuracy and
sensitivity are unmatched by conventional analytical techniques. Identification of proteins and characterisation of their
primary structure is a rapidly growing field in the post-genomic era, where matrix-assisted laser desorption/ionisation time-of-flight
peptide mass fingerprinting combined with electrospray tandem mass spectrometry can efficiently solve many questions. Many
recently determined genomic sequences have not been characterised at the protein level. Analysis of the amino acid sequence
and characterisation of post-translational modifications are therefore important steps towards correlation of protein structure
with function. This review concerns methods, instrumentation and applications of mass spectrometry in protein and peptide
analysis.
Received 17 April 2001; accepted 19 April 2001 |
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Keywords: | , Mass spectrometry, protein, peptide, matrix-assisted laser desorption/ionisation, electrospray ionisation, quadrupole,,,,,,time-of-flight, |
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