| 血管生成抑制因子arresten克隆及功能的初步研究 |
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| 引用本文： | 王鑫,陈佩林,倪晓华,曹根涛,周丹.血管生成抑制因子arresten克隆及功能的初步研究[J].南通大学学报(自然科学版),2007,6(1):47-51. |
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| 作者姓名： | 王鑫 陈佩林 倪晓华 曹根涛 周丹 |
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| 作者单位： | 华东师范大学生命科学学院,上海,200062;上海新智生物技术有限公司,上海,200031 |
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| 摘 要： | 血管生成抑制因子arresten是血管生成及肿瘤生长抑制物.研究发现,将arresten基因重组于质粒pGEX4T1转化入大肠杆菌BL21并用IPTG诱导蛋白表达,可获得分子量为45kDa的目的蛋白条带,arresten蛋白多数以包涵体形式存在,也有可溶蛋白存在于菌裂解液的上清,可溶蛋白可由Sepharose 4B凝胶柱亲和层析柱纯化.在鸡胚绒毛尿囊膜(chick chorioallantoic membrane,CAM)实验中,arresten基因可溶蛋白被证实抑制新生血管生成的效果较好.
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| 关 键 词： | arresten基因 质粒构建 鸡胚绒毛尿囊膜法 |
| 文章编号： | 1673-2340（2007）01-0047-05 |
| 修稿时间： | 2006-06-05 |
Preliminary Study on Cloning and Functions of Human Arresten Gene |
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| Authors: | WANG Xin CHEN Pei-lin NI Xiao-hua CAO Gen-tao ZHOU Dan |
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| Institution: | 1. School of Life Sciences, East China Normal University, Shanghai 200062, China; 2.Shanghai Xinzhi Biological technology Co.Ltd, Shanghai 200031,China |
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| Abstract: | Arresten has been identified as an inhibitor of angiogenesis and tumor growth. In this report, the arresten gene was cloned into pGEX4T1 vector and arresten expression was induced by IPTG in E.coli BL21. SDS-PAGE analysis indicated that the expressed pattern was about 45 kDa. Arresten protein existed mainly as inclusion body, less as dissoluble body. The later one can be purified though the Glutathione Sepharose 4B gel column without any denaturalization to insure its biology activity. CAM (chick chorioallantoic membrane) assay showed that arresten could effectively inhibit the angiogenesis of chick chorioallantoic membrane vessel. |
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| Keywords: | Human arresten gene vector construction CAM assay |
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