Butanol extracts from myelin fragments. III. SDS-urea gel electrophoretic studies

Summary The butanol extracts from myelin fragments showing 5-HT binding capacity were studied by SDS-urea gel electrophoresis. 5 main bands were observed and their molecular weights were determined by the method of Ferguson relationship, and revealed that these extracts contained proteolipid protein, DM-20 and basic proteins. Furthermore, the reconstituted fraction with crude basic proteins and lipids showed the saturable binding capacity for C¹⁴·5-HT.Acknowledgment. We thank Prof. T. Iwamoto for his encouragement during the course of this study.