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| 基因芯片制作中玻璃介质表面处理对DNA固定率的影响 | |
| 引用本文： | 祝骥,马文丽,李凌,姚汝华,郑文岭.基因芯片制作中玻璃介质表面处理对DNA固定率的影响[J].华南理工大学学报(自然科学版),2002,30(3):94-97. |
| 作者姓名： | 祝骥 马文丽 李凌 姚汝华 郑文岭 |
| 作者单位： | 1. 华南理工大学,生物工程系,广东,广州,510640 2. 第一军医大学,分子生物学研究所,广东,广州,510515 3. 广州军区总医院分子肿瘤研究所,广东,广州,510010 |
| 摘 要： | 用显微镜载玻片作为基因芯片制作中DNA固定介质材料，对玻片表面分别进行醛基化、异硫氰酸基化、氨基化等不同修饰处理，分析其对DNA固定率的影响，并比较了国产与进口玻片处理间的差异。结果表明，异硫氰酸基化处理玻片DNA固定率高于醛基化、氨基化处理，接近进口的氨基化玻片，但不同处理间及国产与进口玻片间差异不显著。 |
| 关 键 词： | 基因芯片 玻璃介质 表面处理 固定率 DNA 基因固定 醛基化 异硫氰酸基化 |
| 文章编号： | 1000-565X(2002)03-0094-04 |
| 修稿时间： | 2000年8月30日 |
Effects of Glass Slide Surface Treatment on DNA Attachment Rate in the Fabrication of Genechip |
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| Zhu Ji,Ma Wen_li,Li Ling,Yao Ru_hua,Zheng Wen_ling.Effects of Glass Slide Surface Treatment on DNA Attachment Rate in the Fabrication of Genechip[J].Journal of South China University of Technology(Natural Science Edition),2002,30(3):94-97. | |
| Authors: | Zhu Ji Ma Wen_li Li Ling Yao Ru_hua Zheng Wen_ling |
| Institution: | Zhu Ji 1 Ma Wen_li 2 Li Ling 2 Yao Ru_hua 1 Zheng Wen_ling 3 |
| Abstract: | The experiment uses glass slides made in China or imported as DNA attachment supports, and modifies the surface with aldehydation, isothiocyanic acidation or amino_group. Effects of the treatments on the DNA attachment rate and difference between the two kinds of glass slides are analyzed. The result shows that the DNA attachment rate of slides which was treated with isothiocyanic acidation is higher than those of the other two treatments, and is close to the amino_group modified glass slides, but the difference among the different treatments and between the slides made in China and the slides imported is not remarkable. |
| Keywords: | genechip glass slide surface treatment attachment rate DNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |