| 毛细管区带电泳富集分离烟草生物碱 |
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| 引用本文： | 许庆平,王蕾,何友昭.毛细管区带电泳富集分离烟草生物碱[J].中国科学技术大学学报,2006,36(10):1096-1100,1126. |
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| 作者姓名： | 许庆平 王蕾 何友昭 |
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| 作者单位： | 中国科学技术大学化学系,安徽合肥,230026 |
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| 摘 要： | 采用pH 3.2,360 mmol/L酒石酸缓冲体系,毛细管区带电泳能成功地将烟草生物碱基线分离.与磷酸缓冲液相比,该方法提高了分离度和检测灵敏度.米喔斯明的分离度和检出限分别为1.6和2 ng/mL.此外,还研究和采用了卷烟样品中痕量生物碱的毛细管电泳阳离子选择性耗尽在柱富集方法.
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| 关 键 词： | 毛细管区带电泳 酒石酸缓冲液 烟草生物碱 阳离子选择性耗尽注入 |
| 文章编号： | 0253-2778（2006）10-1096-05 |
| 收稿时间： | 2006-03-17 |
| 修稿时间： | 2006-03-172006-06-22 |
Preconcentration and separation of tobacco alkaloids by capillary zone electrophoresis |
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| XU Qing-ping,WANG Lei,HE You-zhao.Preconcentration and separation of tobacco alkaloids by capillary zone electrophoresis[J].Journal of University of Science and Technology of China,2006,36(10):1096-1100,1126. |
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| Authors: | XU Qing-ping WANG Lei HE You-zhao |
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| Institution: | Department of Chemistry, University of Science and Technology of China, Hefei 230026, China |
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| Abstract: | Tobacco alkaloids were separated on baseline by capillary zone electrophoresis with a buffer solution of pH 3.2, 360 mmol/L tartaric acid. Cation-selective exhaustive injection, as an on-column preconcentration method, was also investigated for the determination of the alkaloids of cigarette samples. With the proposed method, both the resolution and detection limit of the alkaloids were improved comparing with those in phosphate buffer. The resolution and detection limit of myosmine was 1.6 and 2 ng/mL, respectively. |
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| Keywords: | capillary zone electrophoresis tartaric acid buffer tobacco alkaloids cation-selective exhaustive injection |
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