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| 黄牛与水牛的毛状肉孢子虫同工酶研究 | |
| 引用本文： | 梁亦龙,谢永芳,舒坤贤,左仰贤,谭德勇,陈新文.黄牛与水牛的毛状肉孢子虫同工酶研究[J].重庆邮电大学学报(自然科学版),2006,18(2):276-278. |
| 作者姓名： | 梁亦龙 谢永芳 舒坤贤 左仰贤 谭德勇 陈新文 |
| 作者单位： | 1. 重庆邮电学院,重庆,400065;云南大学,生命科学学院,云南,昆明,650091 2. 重庆邮电学院,重庆,400065 3. 云南大学,生命科学学院,云南,昆明,650091 |
| 基金项目： | 重庆邮电学院校科研和教改项目 |
| 摘 要： | 采用聚丙烯酰胺垂直平板梯度凝胶电泳的方法,对寄生于黄牛和水牛的毛状肉孢子虫进行同工酶比较研究,探讨肉孢子虫的宿主特异性.结果表明黄牛与水牛的毛状肉孢子虫同工酶与工酶谱非常相似,只在活性上略有差异,酶谱存在不同程度的种间差异可作为黄牛与水牛的毛状肉孢子虫同工酶的遗传鉴定标记.从分子水平证明黄牛和水牛都是毛状肉孢子虫自然中间宿主. |
| 关 键 词： | 毛状肉孢子虫 同工酶 等电聚焦 电泳 |
| 文章编号： | 1004-5694(2006)02-0276-03 |
| 收稿时间： | 2005/5/13 0:00:00 |
| 修稿时间： | 2005年5月13日 |
Isozyme study on sarcocystis hirsuta of cattle and water buffalo |
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| LIANG Yi-long,XIE Yong-fang,SHU Kun-xian,ZUO Yang-xian,TAN De-yong,CHEN Xin-wen.Isozyme study on sarcocystis hirsuta of cattle and water buffalo[J].Journal of Chongqing University of Posts and Telecommunications,2006,18(2):276-278. | |
| Authors: | LIANG Yi-long XIE Yong-fang SHU Kun-xian ZUO Yang-xian TAN De-yong CHEN Xin-wen |
| Institution: | Chongqing University of Posts and Communications, Chongqing 400065,P.R.China |
| Abstract: | This research concerns about the isozymes analysis of parasitic sarcocystis hirsuta from water buffalo and cattle by means of isoelectric focusing electrophoresis to explore the host specificity of sarcocystis species. Different enzymes were extracted from each of samples. All isolates of S. hirsuta from infected water buffalo and cattle showed identical enzyme profiles. The data support the hypothesis that both water buffalo and cattle are natural intermediate hosts of S. hirsuta at the level of gene, since isozyme is the biochemical phenotype of gene. |
| Keywords: | sarcocystis hirsuta isozyme isoelectric focusing electrophoresis |
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