| 培养因子对绿玉树离体微型快繁的影响 |
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| 引用本文： | 蒋丽娟,孙友平,李培旺.培养因子对绿玉树离体微型快繁的影响[J].广西科学,2003,10(3):232-237. |
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| 作者姓名： | 蒋丽娟 孙友平 李培旺 |
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| 作者单位： | 1. 湖南省林业科学院,长沙市,410004
2. 华中科技大学生命科学院,武汉,430074 |
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| 基金项目： | 国家林业局“948”项目，“863”项目《能源植物及液体燃料利用新技术研究示范》资助。 |
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| 摘 要： | 以绿玉树的腋芽茎段和顶芽为外植体，研究不同培养基浓度、不同激素配比、外植体的放置方式以及化学物质PEG、PP333、AgN03等因子对绿玉树离体培养丛生芽产生的影响，以此筛选出绿玉树离体快繁最适培养基及培养途径。结果表明：诱导腋芽、顶芽萌发较理想的培养基为：3／4MS ZT0．5～1mg／L NAA0．01mg／L和1／2MS BA0.5mg／L NAA0．01mg／L，适宜丛生芽增殖的培养基为：MS ZT0．5～2mg／L NAA0．05mg／L GA30.01mg／L，芽的增殖系数为4．5。
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| 关 键 词： | 绿玉树 微型快繁 培养基 增殖系数 |
| 收稿时间： | 2003/5/27 0:00:00 |
| 修稿时间： | 2003年5月27日 |
Effects of Culture Factors on Micro-propagation of Euphorbia tirucalli |
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| Jiang Lijuan,Sun Youping and Li Peiwang.Effects of Culture Factors on Micro-propagation of Euphorbia tirucalli[J].Guangxi Sciences,2003,10(3):232-237. |
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| Authors: | Jiang Lijuan Sun Youping and Li Peiwang |
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| Institution: | Hunan Academy of Forestry, Changsha, Hunan, 410004, China,Hunan Academy of Forestry, Changsha, Hunan, 410004, China and Hunan Academy of Forestry, Changsha, Hunan, 410004, China |
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| Abstract: | The effects of contents of mediums,phytohormone,position of explant on the mediums and the chemical such as PEG,PP 333,AgNO 3 on the regeneration of cluster buds were studied by using the stem fragment with auxiliary buds and shoot tip as explants.The results show that mediums of 3/4MS+ZT0.5~1 mg/L+NAA0.01mg/L and 1/2MS+BA0.5mg/L+NAA0.01mg/L,were suitble for the geminating of shoot tips and auxiliary buds.Mediums of MS+ZT0.5~2mg/L+NAA0.05mg/L+GA 3 0.01mg/L was optimum for reproducing cluster buds,and the coefficient of plantlet regeneration was up to 4.5. |
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| Keywords: | Euphorbia tirucalli micro-propagation mediums regeneration coefficient |
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