| 拟南芥耐干旱相关转录因子DREB1A基因的克隆及序列分析 |
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| 引用本文： | 李莉,吴秀玲. 拟南芥耐干旱相关转录因子DREB1A基因的克隆及序列分析[J]. 河南师范大学学报(自然科学版), 2006, 34(1): 117-120 |
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| 作者姓名： | 李莉 吴秀玲 |
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| 作者单位： | 河南师范大学,生命科学学院,河南,新乡,453007;山东省济宁职业技术学院,生化系,山东,济宁,272037 |
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| 摘 要： | 设计引物利用PCR技术从拟南芥中克隆与植物耐旱相关序列DRE相结合的转录因子DREB1A的基因,得到扩增谱带后,进行TA克隆,经蓝白斑筛选获得pDREB重组质粒,经PCR验证和序列测定确认扩增所得片段为DREB1A基因,并对该基因序列进行了分析.
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| 关 键 词： | 拟南芥 DREB1A转录因子 基因克隆 序列分析 |
| 文章编号： | 1000-2367(2006)01-0117-04 |
| 收稿时间： | 2005-08-03 |
| 修稿时间： | 2005-08-03 |
Cloning and Sequence Analysis of Transcription Factor DREB1A Gene Related to Drought Tolerance from Arabidopsis |
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| LI Li,WU Xiu-ling. Cloning and Sequence Analysis of Transcription Factor DREB1A Gene Related to Drought Tolerance from Arabidopsis[J]. Journal of Henan Normal University(Natural Science), 2006, 34(1): 117-120 |
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| Authors: | LI Li WU Xiu-ling |
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| Affiliation: | 1. College of Life Science, Henan Nomal University, Xinxiang 453007,China; 2. Department of Biochemical Engineering, Jining Vocational Technology College, Jining 272037, China |
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| Abstract: | A pair of designed primers were used to clone DREB1A gene from Arabidopsis,which was related to the plant tolerance of the drought stress. After a bright band was acquired, it was inserted into TA cloning vector. Then we got recombinant plasmid by white/blue screening. And the gene was confirmed and analysised through PCR testification and sequenceing. |
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