温阳抗寒合剂治疗支气管哮喘小鼠作用机制实验研究

目的观察温阳抗寒合剂(WYKHM)对哮喘小鼠的治疗作用及机制.方法 60只Balb/c小鼠随机分为哮喘组(Ast)、氨茶碱组(Ami)、WYKHM高中低3个剂量组(200 mg/kg,100 mg/kg,50 mg/kg)和空白对照组(Con).卵清蛋白(OVA)使小鼠致敏,1周后灌胃给药,2周后OVA诱发哮喘,48 h后眶静脉取血,用D-Hank液灌洗支气管肺泡(BALF),测定BALF中白细胞分类计数、白介素-5(IL-5)、白介素-23(IL-23)含量、一氧化氮(NO)、一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)和丙二醛(MDA)水平.结果 WYKHM能显著降低BALF中NO和NOS的含量,与哮喘组比较差异具有统计学意义(P0.05或P0.001);WYKHM和氨茶碱组BALF中IL-5,IL-23含量均低于哮喘组(P0.05或P0.01);治疗组BALF中SOD活性明显上升,MDA水平明显下降,与对照组和哮喘组比较差异具有统计学意义(P0.05或P0.01).结论 WYKHM可降低NOS活性,降低NO水平;WYKHM可降低IL-5和IL-23含量;WYKHM可提高SOD对氧自由基等毒性物质的清除能力,还可减少MDA水平.

Experimental Study on Effect Mechanism of Wen Yang Kan Han Mixture on Bronchial Asthma in Mice

Objective To observe the therapeutic effect and underlying mechanism of traditional Chinese preparation Wen Yang Kang Han Mixture (WYKHM)on bronchial asthma in mice. Method Sixty Balb/c mice were randomized into six groups,asthmatic group,aminophylline group,control group,high dose group (200 mg/kg),middle dose group (100 mg/kg) and low dose group (50 mg/kg). The mice were sensitized by injecting ovalbumin except the control group and the experimental groups were administrated WYKHM after 1 week. After 2 weeks,ovalbumin was given to the mice to induce asthma. Then,the blood was taken from orbital vein in all the mice after 48 hours. D-Hanks balanced liquor was lavaged into bronchial alveolus to draw BALF. The concentrations of IL-5,IL-23,NO, NOS,SOD and MDA in BALF were measured. Results Compared with the asthmatic group,WYKHM decreased the contents of NO and NOS in BALF dramatically (P<0. 05 or P<0. 001). IL-5 concentrations of WYKHM groups and aminophylline group were lower than that of the asthmatic group with obvious difference ( P<0. 05 or P<0. 001). Compared with the control group and asthmatic group,SOD levels and MDA levels of the treatment groups were significantly higher and lower,respectively (P<0. 05 or P<0. 01). Conclusion WYKHM could alleviate asthmatic symptoms by decreasing NO level, MDA level and NOS activity, by elevating SOD content, and by regulating the key factor of IL-5 and IL-23.