Methods of experimental yolk removal fromBrachydanio rerio eggs

Summary The yolk ofBrachydanio rerio eggs was removed by pipettation, bursting and cutting off with a scalpel. The total yolk removal before the 8-cell stage led to a germ with irregular groups of cells, at the 8-cell stage to a topologically irregular differentiation, at the 64-cell stage to a nearly normal embryo, which is not viable.