| 用液氮淬冷法进行花粉破壁的实验研究 |
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| 引用本文： | 周顺华,陶乐仁,徐斐,刘宝林,郭旭峰.用液氮淬冷法进行花粉破壁的实验研究[J].上海理工大学学报,2002,24(3):233-237. |
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| 作者姓名： | 周顺华 陶乐仁 徐斐 刘宝林 郭旭峰 |
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| 作者单位： | 上海理工大学,动力工程学院,上海,200093 |
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| 摘 要： | 通过正交设计实验，利用液氮将油菜花粉进行深低温超快速冷冻处理后，借助显微镜对解冻后的花粉进行显微观察，拍照和破壁率统计，同时采用苯酚-硫酸法进行胞外总糖含量测定，实验表明油菜花粉破壁优化条件为：外包紫铜，解冻温度95℃，反复冻结解冻3次，此时每克花粉中胞外总糖变化量最大值达59.588mg(以葡萄糖计），较未经冻结解冻处理的上升了31.25%，破壁率达94.97%.
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| 关 键 词： | 油菜花粉 破壁率 液氮 正交实验 苯酚－硫酸法 显微观察 |
| 文章编号： | 1007-6735(2002)03-0233-05 |
| 修稿时间： | 2001年11月28 |
Experimental study on pollen breaking by quenching into liquid nitrogen |
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| ZHOU Shun-hu,TAO Le-ren,XU Fei,LIU Bao-lin,GUO Xu-feng.Experimental study on pollen breaking by quenching into liquid nitrogen[J].Journal of University of Shanghai For Science and Technology,2002,24(3):233-237. |
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| Authors: | ZHOU Shun-hu TAO Le-ren XU Fei LIU Bao-lin GUO Xu-feng |
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| Abstract: | A new simple but effective method for breaking pollen wall by quenching into liquid nitrogen is proposed in the paper. As the result of microscope observation, breaking ratio calculation and phenol/sulphuric reaction, the maximum yield of total sugar is up to 59.588 milligram per gram of pollen(based on glucose) under the following conditions: packaged with sheet copper; defrozen at 95; frozen and defrozen for 3 times, which increases by 31.25 percent over the unprocessed one, with a high breaking ratio of 94.97%. |
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| Keywords: | cole pollen breaking ratio liquid nitrogen orthogonal experiment microscope observation phenol/sulphuric reaction |
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