| 共价修饰的辣根过氧化物酶及其在酶传感器中的应用 |
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| 引用本文： | 王雅琴.共价修饰的辣根过氧化物酶及其在酶传感器中的应用[J].北京化工大学学报(自然科学版),1999,26(1). |
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| 作者姓名： | 王雅琴 |
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| 作者单位： | 北京化工大学化学工程学院,北京,100029 |
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| 摘 要： | 将介体对甲酰苯基二茂铁（ＦＰＦ）与辣根过氧化物酶（ＨＲＰ）共价结合，然后同单体吡咯一起电聚合到铂电极上，再通过戊二醛将葡萄糖氧化酶（ＧＯＤ）交联固定在电极上，制成共价修饰的ＨＲＰ／ＧＯＤ介体酶传感器。共价修饰的酶传感器响应电流增大，底物测试范围０～４０ｍｍｏｌ／Ｌ，电极稳定性增强。
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| 关 键 词： | 辣根过氧化物酶 共价修饰 二茂铁 酶传感器 |
Covalent modification of horseradish peroxidase and its application in enzyme sensor |
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| Wang Yaqin.Covalent modification of horseradish peroxidase and its application in enzyme sensor[J].Journal of Beijing University of Chemical Technology,1999,26(1). |
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| Authors: | Wang Yaqin |
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| Abstract: | Horseradish peroxidase (HRP) was modified chemically with p formyl phenylferrocene (FPF). The modified HRP and pryyole were electrochemically polymerized and glucose oxidase (GOD) was immobilized chemically with glutaraldehyde onto platinum wire electrode to form HRP/GOD biosensor. Direct electrical communication can be established between the modified HRP and metal electrode. The enzyme sensor was used for determination of the concentration of hydrogen and glucose, which result in largely improving the response of the enzyme sensor to glucose. |
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| Keywords: | horseradish peroxidase covalent modification ferrocene enzyme sensor |
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