| AtOSAK1在植物氧化胁迫响应中的功能研究 |
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| 引用本文： | 邓伟玲, 刘译阳, 张盛春, 阳成伟. AtOSAK1在植物氧化胁迫响应中的功能研究[J]. 华南师范大学学报（自然科学版）, 2015, 47(3): 86-0. DOI: 10.6054/j.jscnun.2015.03.011 |
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| 作者姓名： | 邓伟玲 刘译阳 张盛春 阳成伟 |
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| 作者单位： | 1.华南师范大学生命科学学院 |
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| 基金项目： | 国家自然科学基金项目(U1201212) |
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| 摘 要： | 植物类蛋白激酶ABC1家族(Activity of bc1 complex)在植物生长发育及响应非生物胁迫中扮演着重要的作用。我们克隆并研究了ABC1家族中一个的基因AtOSAK1。半定量RT-PCR及启动子驱动GUS报告基因分析显示，AtOSAK1基因的表达无组织特异性，并且主要受甲基紫精(MV)的诱导表达。AtOSAK1-GFP亚细胞定位显示，AtOSAK1是一个定位在叶绿体中的蛋白。不同浓度MV处理条件下，过表达AtOSAK1植株的种子萌发率和存活率比野生型(Col-0)和突变体osak1高。这些研究表明，AtOSAK1基因参与了抗氧化胁迫的响应.
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| 关 键 词： | 氧化胁迫 |
| 收稿时间： | 2015-03-09 |
| 修稿时间： | 2014-09-17 |
Functional Analysis of AtOSAK1 Gene in Response to Oxidative Stress in Arabidopsis |
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| Functional Analysis of AtOSAK1 Gene in Response to oxidative stress in Arabidopsis[J]. Journal of South China Normal University (Natural Science Edition), 2015, 47(3): 86-0. DOI: 10.6054/j.jscnun.2015.03.011 |
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| Authors: | Deng Weiling Liu Yiyang Zhang Shengchun Yang Chengwei |
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| Affiliation: | Deng Weiling;Liu Yiyang;Zhang Shengchun;Yang Chengwei;Guangdong Key Lab of Biotechnology for Plant Development,College of Life Science,South China Normal University; |
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| Abstract: | A typical kinase family, ABC1 (the activity of bc1 complex) genes play essential roles in plant growth, development and responses to be environmental stresses. We cloned and characterized a gene of the ABC1 family in Arabidopsis. Semi-quantitative RT-PCR and GUS staining analysis revealed that it was constitutively expressed and mainly induced under methyl viologen (MV) treatment. AtOSAK1-GFP fusion analysis showed that AtOSAK1 is localized in chloroplasts. Transgenic plants overexpressing AtOSAK1 showed higher rates of seed germination and seedling survival to MV stress than that of the Col-0 and the knock-out mutant osak1. These results suggested that AtOSAK1 may be involved in response to oxidative stress in Arabidopsis. |
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| Keywords: | Arabidopsis typical kinase ABC1 methyl viologen oxidative stress |
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