藏红花内生真菌的分离和代谢产物的初步研究

通过不同灭菌处理方式和4种不同培养基培养，从健康藏红花植株体内分离得到63株内生真菌．其中，球茎中分离到60株内生真菌，花药中分离到2株，柱头中分离到1株，花被、嫩叶、芽鞘、花柱中均未分离到内生真菌．4种培养基的筛选结果表明：高盐察氏培养基相对适合于藏红花内生真菌的分离．通过对4种病源指示菌的生长抑制试验，筛选出12株内生真菌对病原菌有一定程度的抑制作用．通过对分泌物产生菌的代谢产物分析研究，发现4株特异性代谢黄酮类或皂甙类物质的生产菌．

The initial research on osolation and metabolism of endophytic fungus from Crocus sativus L.

After sterilized with different methods followed by culture on four kinds of media,sixty-three endophytic fungal strains were isolated from healthy Crocus sativus L.,among which sixty from bulbs,two from anthers,one from stigmas.However no fungal strains were isolated from perianth,leaves,coleoptiles and styles.The screening results for culture mediums show that the high-salt czapeks'medium is suitable for culture of endophytic fungus.The antifungal activity screening with four microbe show that twelve endo...