| 导入脂肪酶基因提高荧光假单胞菌26-2的产酶效率 |
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| 引用本文： | 张搏,王青艳. 导入脂肪酶基因提高荧光假单胞菌26-2的产酶效率[J]. 广西科学, 2009, 16(2): 185-187 |
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| 作者姓名： | 张搏 王青艳 |
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| 作者单位： | 广西科学院,广西南宁,530007;广西科学院,广西南宁,530007 |
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| 摘 要： | 采用导入脂肪酶基因的方法对荧光假单胞菌（Pseudomonas fluorescence）26—2的产酶效率进行实验。实验以质粒ppic9k—lipA为模板，通过PCR的方式在26—2脂肪酶基因的两端引入BamHl和EcoR1的酶切位点，并将该基因与大肠杆菌荧光假单胞菌穿梭质粒pDSK 519连接，获得重组质粒pDSK519-lipA，再将重组质粒转入荧光假单胞菌26—2，获得工程菌株P．fluorescence 26—2—1。在相同的发酵条件下，工程菌株的发酵液酶活力比原始菌株的发酵液酶活力提高2倍，实现了荧光假单胞菌脂肪酶基因的同源性表达。
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| 关 键 词： | 荧光假单胞菌 同源性表达 脂肪酶基因 |
| 收稿时间： | 2008-11-17 |
Improving the Lipase Production of Pseudomonas fluorescence 26-2 through Transform Its Lipase Gene into the Originally Strain |
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| ZHANG Bo and WANG Qing-yan. Improving the Lipase Production of Pseudomonas fluorescence 26-2 through Transform Its Lipase Gene into the Originally Strain[J]. Guangxi Sciences, 2009, 16(2): 185-187 |
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| Authors: | ZHANG Bo and WANG Qing-yan |
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| Affiliation: | Guangxi Academy of Science;Nanning;Guangxi;530007;China |
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| Abstract: | A Pseudomonas fluorescence lipase gene lipA was subcloned from ppic9k-lipA and inserted into vector pDSK519 to construct a new plasmid,pDSK519-lipA,which was transformed into its original strain P.fluorescence 26-2.The recombinant strain 26-2-1 was confirmed by restriction digestion.Compared with wild type 26-2 in pot experiment and ferment experiment,the lipase production of 26-2-1 is 2 times more than its original strain |
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| Keywords: | P.fluorescence homologous expression lipase gene |
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