Demonstration of lateral asymmetry in G-banded mouse embryo chromosomes |
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| Authors: | S M Tucci R G Skalko |
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| Institution: | (1) Department of Anatomy, Albany Medical College Albany, and Birth Defects Institute, New York State Health Department, and Institute of Comparative and Human Toxicology, Albany Medical College, 12237 Albany, New York, USA;(2) Present address: Department of Anatomy, College of Medicine, East Tennessee State University, 37601 Johnson City, Tennessee, USA |
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| Abstract: | Summary Cells obtained from 10-day mouse embryos were cultured for 3 h in medium containing colcemid. Chromosome preparations were subjected to G-banding in either calcium-magnesium free Hank's solution or the ASG method. From one to several occurrences of lateral asymmetry (unequal banding of sister chromatids) were observed in the majority of karyotypes analyzed.Partial support for this study was provided by grant no. 2-PO1-ES00226-10, NIH, NIEHS and by a grant from the National Foundation-March of Dimes. We thank Don Driscoll for his capable assistance. |
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