Detection of theH-RAS oncogene in human thyroid anaplastic carcinomas |
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| Authors: | B. M. Stringer J. M. Rowson M. H. Parkar J. M. Seid P. R. Hearn D. Wynford-Thomas S. Ingemansson N. Woodhouse Dr. M. H. Goyns |
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| Affiliation: | (1) Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, P.O. Box 3354, 11211 Riyadh, (Saudi Arabia);(2) Department of Surgery, King Faisal Specialist Hospital and Research Centre, P.O. Box 3354, 11211 Riyadh, (Saudi Arabia);(3) Department of Medicine, King Faisal Specialist Hospital and Research Centre, P.O. Box 3354, 11211 Riyadh, (Saudi Arabia);(4) Unit of Cancer Biology, Department of Pathology University Hospital of Wales, Heath, CF4 4XN Cardiff, (England);(5) Department of Clinical Oncology, Royal Hallamshire Hospital, S 10 2JF Sheffield, England |
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| Abstract: | Summary We have transfected high-molecular-weight DNA from human thyroid carcinomas into murine 3T3 cells. As a result we identified several foci of morphologically distinct transformed cells in each of the tumour DNA transfected cultures. After a total of three rounds of transfection, the transformed cells were shown to form tumours in nude mice. Southern blot analysis of DNA prepared from third-round transfectants demonstrated the presence of human Alu repetitive sequences and, after hybridization with probes for known oncogenes, indicated the presence of the humanH-RAS oncogene in 3T3 cells transfected with three out of four anaplastic carcinoma DNA samples. It appears therefore that activation ofRAS genes may be an important event in the development of the anaplastic thyroid tumours. |
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| Keywords: | Anaplastic carcinoma thyroid H-RAS oncogene transfection |
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