新鲜羊膜移植对碱烧伤早期角膜及房水中超氧化物歧化酶活力，丙二醛含量的影响

目的：探讨新鲜羊膜移植在早期角膜碱烧伤治疗中与自由基反应的关系，方法：以48只新西兰大白兔耕作角膜碱烧伤模型，烧伤兔被分为新鲜羊膜移植组，保存羊膜移植组及对照组，烧伤后24h分别实施手术，分别于术后14，30d处死实验兔取材房水及角膜组织，检测其超氧化物歧化酶活力及丙二醛含量，结果：新鲜羊膜移植组，保存羊膜移植组丙二醛含量均代于对照组（P＜0．01），超氧化物歧化酶活力均高于对照组（P＜0．01），术后14天新鲜羊膜移植组角膜超氧化物歧化酶活力高于保存羊膜组（P＜0．05），术后30d新鲜羊膜组角膜及房水丙二醛含量的均低于保存羊膜组（P＜0．05），结论：新鲜羊膜移植在早期角碱烧伤治疗中能减轻自由反应对眼组织的损伤，其效果优于保存羊膜移植。

The effects of fresh amniotic membrane transplantation of early alkali burn on the vitality of superoxide dismutase and the malondialdehyde contents in corneas and aqueous

Aim: To find the effect of amniotic membrane transplantation of early alkali burn and its relationship with free redical reaction. Methods: Corneal alkali- burn model of 48 New Zealand white rabbits was made. The burned rabbits were divided into fresh amniotic membrane transplantation (fAMT), preserved amniotic membrane transplantation (pAMT) and control group (C). 24 hours after burn, the operation was adopted.14 days and 30 days after operation,the vitality of Superoxide Dismutase (SOD) and the contents of Malondialdehyde (MDA ) in corneas and aqueous were determined. Results: The contents of MDA in pAMT and fAMT were lower than that in C, and the vitality of SOD were higher.( P <0.01). 14 days after operation, the vitality of SOD in fAMT in corneas was higher than that in pAMT ( P <0.05). 30 days after operation, the contents of MDA in fAMT in corneas and aqueous was lower than that in pAMT ( P <0.05 ). Conclusion: Fresh amniotic membrane transplantation is better than preserved amniotic membrane transplantation; it can lighten the damage to the eyes from free redical reaction for early corneal alkali burn