| 溴甲酚紫分光光度法测定烟草薄片中烟碱的研究 |
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| 引用本文： | 罗勇,王淑敏,刘莉,张立科.溴甲酚紫分光光度法测定烟草薄片中烟碱的研究[J].许昌师专学报,2012(2):97-100. |
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| 作者姓名： | 罗勇 王淑敏 刘莉 张立科 |
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| 作者单位： | [1]河南中烟工业有限责任公司驻马店卷烟厂,河南驻马店463000 [2]许昌学院化学化工学院,河南许昌461000 |
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| 基金项目： | 基金项目：许昌学院科研基金计划项目（20118022） |
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| 摘 要： | 在HAc-NaAc缓冲溶液中,研究了溴甲酚紫与烟碱的相互作用,烟碱-溴甲酚紫-Co2＋三元络合物的最大吸收波长λmax=436 nm,摩尔吸光系数κ=6.473 5 L·μmol-1·cm-1,烟碱浓度在0.20～100.00μmol·L-1范围内符合比尔定律,线性回归方程Y=0.647 4X＋0.009 4,相关系数R=0.999 6.根据信噪比确定检测限（S/N=3,n=7）为0.10μmol·L-1,该方法准确度、灵敏度高,选择性好,特别适合于中、小型企业快速、大批量地分析烟草样品中的烟碱.
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| 关 键 词： | 紫外可见 烟碱 溴甲酚紫 Co2＋ 增敏 十二烷基磺酸钠 |
Study on the Determination of Nicotine in Reconstituted Tobacco using Bromcresol Purple Spectrophotemetry |
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| LUO Yong,WANG Shu-min,LIU Li,ZHANG Li-ke.Study on the Determination of Nicotine in Reconstituted Tobacco using Bromcresol Purple Spectrophotemetry[J].Journal of Xuchang Teachers College(Social Science Edition),2012(2):97-100. |
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| Authors: | LUO Yong WANG Shu-min LIU Li ZHANG Li-ke |
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| Institution: | 1. China Tobacco Henan Industrial Co. , LTD, Zhumadian Cigarettes Factory, Zhumadian 463000, China ; 2. College of Chmistry and Chmical Engneering, Xuchang University, Xuchang 461000, China) |
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| Abstract: | The interaction between bromcresol purple and nicotine in the HAc-NaAc buffer solution was studied. When the maximum absorption wavelength of nicotine-bromocresol purple - Co2＋ ternary complex Amax = 436 nm, the molar absorption coefficientκ=6.473 5 L·μmol-1·cm-1, Bear＇s law is obeyed by the concentra- tion of nicotine in the range of 0.20 -100.00 μmol·L-1, the linear regression equation is Y = 0. 647 35X ＋ 0. 009 4, the correlation coefficient is R = 0. 999 6, the detection limit is 0.01 μmol·L-1. This method has high accuracy, sensitivity and selectivity, especially suitable for the quick and mass determination of nicotine in tobacco samples in small and medium enterprises. |
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| Keywords: | UV nicotine bromcresol purple Co2 ＋ sensitization sodium dodecyl sulfate |
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