NREL法测定大米草原料组分的含量

采用美国国家可再生能源实验室(NREL)方法定量大米草原料中纤维素、半纤维素及木质素。72%浓硫酸水解1 h、4%稀硫酸水解1 h可将大米草的纤维素、半纤维素降解为可用HPLC定量的单糖,适宜的样品添加量为0.3 g。同时,NREL法测定大米草纤维素、半纤维素及木质素的含量分别为32.92%、27.65%和24.2%。这三个组分的含量是评价大米草预处理、酶解及发酵工艺条件的重要依据。

DETERMINATION OF SPARTINA ANGLICA STOCK COMPONENTS BY NREL METHOD

Cellulose,hemicellulose and lignin of Spartina anglica were quantified by National Renewable Energy Lab (NREL) method.It was showed that the cellulose and semicellulose of S.anglica could be degraded to mono-sugars which could be quantified by HPLC after it was hydrolyzed with 72% concentrated sulphuric acid for 1 h and 4% dilute sulphuric acid for 1h with the appropriate additive amount of 0.3 g sample.Meanwhile,the contents of cellulose,hemicellulose and lignin of S.anglica determined by NREL method were 32.92%,27.65% and 24.2% respectively.The contents of these three fractions are important basis for the evaluation of the pre-processing,enzymatic hydrolysis and fermentation conditions of S.anglica.