原儿茶酸在大鼠血浆中的药代动力学研究

研究原儿茶酸在大鼠血浆中的药代动力学.以原儿茶酸50、100、150 mg/kg(低、中、高)3剂量对大鼠灌胃给药,血浆样品经甲醇乙腈沉淀蛋白后,用HPLC法测定原儿茶酸的浓度,绘制药时曲线.实验数据用3P97药动学软件处理,得相应药动学参数.原儿茶酸在大鼠体内的药动学过程符合二室开放模型.原儿茶酸在剂量由50 mg/kg增至100 mg/kg时药动学过程呈现出非剂量依赖,具有非线性特征;其剂量由100 mg/kg增至150 mg/kg时药动学过程呈现出剂量依赖,具有线性特征.对于其产生非线性特征的原因,需要通过进一步实验进行研究确证.

The pharmacokinetics of protocatechuic acid in rat plasma for intragastric administration

To investigate the pharmacokinetics of protocatechuic acid in rat plasma.Rats were oral administered protocatechuic acid at three doses of 50,100,150 mg/kg,respectively.Protein precipitation method with methyl acohol and acetonitrile was applied to purify plasma samples.Then the content of protocatechuic acid in plasma samples was determined by HPLC and mean concentration-time curve was drawn.Pharmacokinetic parameters were determined with 3P97 software.Pharmacokinetics of protocatechuic acid in rats could be fitted to two-compartment model.When the dose was between 50 mg/kg and 100 mg/kg,it seemed dose-independent and indicated nonlinear absorption feature.When the dose was between 100 mg/kg and 150 mg/kg,it seemed dose-dependent and indicated linear absorption feature.The reason of nonlinear pharmacokinetic feature should be confirmed in future.