NMR evidence for an early framework intermediate on the folding pathway of ribonuclease A |
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Authors: | J B Udgaonkar R L Baldwin |
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Affiliation: | Department of Biochemistry, Stanford University School of Medicine, California 94305. |
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Abstract: | The presence of an early intermediate on the folding pathway of ribonuclease A has been demonstrated by a study of the exchange reaction between the backbone amide protons in the folding protein and solvent protons using rapid mixing techniques. A structural analysis of the intermediate by two-dimensional 1H-NMR is consistent with the framework model of protein folding in which stable secondary structure first forms the framework necessary for the subsequent formation of the complete tertiary structure. |
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