| 利用农杆菌介导法将抗虫基因导入油菜的研究 |
| |
| 引用本文： | 侯丙凯,黄健.利用农杆菌介导法将抗虫基因导入油菜的研究[J].山东大学学报(理学版),2002,37(5):467-470. |
| |
| 作者姓名： | 侯丙凯 黄健 |
| |
| 作者单位： | 1. 山东大学生命科学学院,济南,250100
2. 青岛教育学院生物系,青岛,266071 |
| |
| 摘 要： | 以油菜的子叶柄作为转化的受体材料,经农杆菌感染和共培养,在含卡那霉素的筛选培养上诱导产了大量的不定芽,经生根培养,获得了再生植株,通过PCR扩增和基因组Southern杂交分析,证明其中5株为转基因植株,对转基因植株进行抗虫性检测,有2株表现出较好的抗虫性。
|
| 关 键 词： | 农杆菌介导法 油菜 土壤农杆菌 抗虫基因 转基因植株 抗虫性 植物基因工程 |
| 文章编号： | 1671-9352(2002)05-0467-04 |
| 修稿时间： | 2002年1月12日 |
Study on the Introduction of Insect- resistant Gene into Oilseedrape Using Agrobacterium tumefaciens- mediated Transformation |
| |
| ZHOU Bing-kai & HUANG Jian,WTBX.Study on the Introduction of Insect- resistant Gene into Oilseedrape Using Agrobacterium tumefaciens- mediated Transformation[J].Journal of Shandong University,2002,37(5):467-470. |
| |
| Authors: | ZHOU Bing-kai & HUANG Jian WTBX |
| |
| Institution: | ZHOU Bing-kai 1 & HUANG Jian 2 WTBX |
| |
| Abstract: | WT5"BZThe cotyledon petiols of oilseed rape WTBX(Brassica napus)WTBZ were used as recipient of transformation. After being infected and co-cultured with WTBXAgrobacterium tumefaciens,WTBZcotyledon petiols produced a lot of adventitious buds on selection medium supplemeted with kanamycin. Kanamycin-resistant plants were then obtained from the adventitious buds by rooting culture. DNA Southern blot hybridization and polymerase chain reaction showed that 5 of the plants were transgenic when probed with the insect-resistant gene. These transgenic plants exhibited different insect-resistant response when they were subjected to leaf feeding experiments, but two of them displayed a good resistance to pest insect larvae. |
| |
| Keywords: | Brassica napus Agrobacterium tumefaciens insect-resistant gene transgenic plant |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
