| 生物合成中低分子量右旋糖酐的初步研究 |
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| 引用本文： | 李廿,;曾平,;唐梅,;李媚,;廖安平. 生物合成中低分子量右旋糖酐的初步研究[J]. 广西民族大学学报, 2014, 0(2): 80-83 |
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| 作者姓名： | 李廿, 曾平, 唐梅, 李媚, 廖安平 |
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| 作者单位： | [1]广西民族大学化学化工学院; [2]广西民族大学广西高校化学与生物转化过程新技术重点实验室,广西南宁530006 |
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| 基金项目： | 广西高校人才小高地建设创新团队项目（桂教[2011]47）;广西科技型中小企业技术创新资金（桂科转13109067）;广西科学研究与技术开发计划项目（桂科能0992028-13）. |
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| 摘 要： | 采用高效液相色谱法对生物法合成中低分子量右旋糖酐的过程进行研究.以肠膜明串珠菌为发酵菌种,发酵得到大分子量右旋糖酐,然后利用圆弧青霉发酵获得的右旋糖酐酶液调控右旋糖酐酶分子量.在选定的条件下,经降解48 h后,发酵液中右旋糖酐分子量约为70 KD～40 KD范围,纯化的降解产物经红外光谱表征,其特征吸收与标准品相同得到中低分子量右旋糖酐.
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| 关 键 词： | 右旋糖酐 高效液相色谱 分子量 合成 降解 |
Preliminary Study on the Biosynthesis of Low Molecular Weight Dextran |
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| Affiliation: | LI Nian, ZENG Ping, TANG Mei, LI Mei, LIAO An-ping (a. School of Chemistry and Chemical Engineering; b. formation Process Technology, Guangxi University for Key Laboratory of Chemical and Biological Trans- Nationalities, Nanning, 530006,China) |
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| Abstract: | The process of low molecular weight dextran biosynthesis was preliminary studied by high performance liquid chromatography（HPLC）. Large molecular weight dextran syntheses with Leuconostoc mesenteroides fermentation were controlled by dextranase which was derived from Penicillium cyclopium. Under the given condition, the range of molecular weight of dextran in fermentation broth was 70 KD-40 KD. In addition, by comparison of the infrared spectra between standard dextran and the purified product, it was confirm that fermentation product and degradation product were exactly dextran. |
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| Keywords: | dextran HPLC molecular weight synthesis degradation |
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